plasmid pkw2 ef sep Search Results


plasmid pkw2 ef sep  (Addgene inc)
93
Addgene inc plasmid pkw2 ef sep
Plasmid Pkw2 Ef Sep, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pkw2+ef+sep/bio_rxiv__2025__07__25__666770-229-9-11?v=Addgene+inc
Average 93 stars, based on 1 article reviews
plasmid pkw2 ef sep - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
e. coli csh26  (Lacpro Industries LLC)
90
Lacpro Industries LLC e. coli csh26
E. Coli Csh26, supplied by Lacpro Industries LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pkw2+ef+sep/pm10373459-92-14-47?v=Lacpro+Industries+LLC
Average 90 stars, based on 1 article reviews
e. coli csh26 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
pcdf 1b nherf1 pdz2 133 300 ser162tag  (Addgene inc)
92
Addgene inc pcdf 1b nherf1 pdz2 133 300 ser162tag
Figure 2. GRK6A binds <t>PDZ1–NHERF1</t> under resting conditions. A, representative immunoblot of NHERF1:GRK6A coIP. HA-GRK6A was cotransfected with either empty vector or the indicated FLAG–NHERF1 construct into OKH cells. WT-NHERF1 coimmunoprecipitates with GRK6A, as does N1P2–GAGA– NHERF1 (PDZ1 intact). N1P1–GAGA <t>(PDZ2</t> intact) and N1P1P2–GAGA/GAGA (both PDZ domains are modified) do not interact with NHERF1. B, NHERF1 coIP with GRK6A was quantified in transfected OKH cells and normalized to WT-NHERF1 (100%). N1P2 interacts with GRK6A to a similar extent to WT-NHERF1. Disruption of PDZ1 (N1P1–GAGA, N1P1P2–GAGA/GAGA) eliminated binding of GRK6A. Results report the mean ± SD (n = 3; ****p < 0.0001, ANOVA). coIP, coimmunoprecipitation; GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1.
Pcdf 1b Nherf1 Pdz2 133 300 Ser162tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pkw2+ef+sep/10__1016_slash_j__jbc__2021__100473-240-12-6?v=Addgene+inc
Average 92 stars, based on 1 article reviews
pcdf 1b nherf1 pdz2 133 300 ser162tag - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier
e coli bl21δ serb cells  (Addgene inc)
92
Addgene inc e coli bl21δ serb cells
Figure 2. GRK6A binds <t>PDZ1–NHERF1</t> under resting conditions. A, representative immunoblot of NHERF1:GRK6A coIP. HA-GRK6A was cotransfected with either empty vector or the indicated FLAG–NHERF1 construct into OKH cells. WT-NHERF1 coimmunoprecipitates with GRK6A, as does N1P2–GAGA– NHERF1 (PDZ1 intact). N1P1–GAGA <t>(PDZ2</t> intact) and N1P1P2–GAGA/GAGA (both PDZ domains are modified) do not interact with NHERF1. B, NHERF1 coIP with GRK6A was quantified in transfected OKH cells and normalized to WT-NHERF1 (100%). N1P2 interacts with GRK6A to a similar extent to WT-NHERF1. Disruption of PDZ1 (N1P1–GAGA, N1P1P2–GAGA/GAGA) eliminated binding of GRK6A. Results report the mean ± SD (n = 3; ****p < 0.0001, ANOVA). coIP, coimmunoprecipitation; GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1.
E Coli Bl21δ Serb Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pkw2+ef+sep/bio_rxiv__2021__03__03__433754-197-11-15?v=Addgene+inc
Average 92 stars, based on 1 article reviews
e coli bl21δ serb cells - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier

Image Search Results


Figure 2. GRK6A binds PDZ1–NHERF1 under resting conditions. A, representative immunoblot of NHERF1:GRK6A coIP. HA-GRK6A was cotransfected with either empty vector or the indicated FLAG–NHERF1 construct into OKH cells. WT-NHERF1 coimmunoprecipitates with GRK6A, as does N1P2–GAGA– NHERF1 (PDZ1 intact). N1P1–GAGA (PDZ2 intact) and N1P1P2–GAGA/GAGA (both PDZ domains are modified) do not interact with NHERF1. B, NHERF1 coIP with GRK6A was quantified in transfected OKH cells and normalized to WT-NHERF1 (100%). N1P2 interacts with GRK6A to a similar extent to WT-NHERF1. Disruption of PDZ1 (N1P1–GAGA, N1P1P2–GAGA/GAGA) eliminated binding of GRK6A. Results report the mean ± SD (n = 3; ****p < 0.0001, ANOVA). coIP, coimmunoprecipitation; GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1.

Journal: Journal of Biological Chemistry

Article Title: Multisite NHERF1 phosphorylation controls GRK6A regulation of hormone-sensitive phosphate transport

doi: 10.1016/j.jbc.2021.100473

Figure Lengend Snippet: Figure 2. GRK6A binds PDZ1–NHERF1 under resting conditions. A, representative immunoblot of NHERF1:GRK6A coIP. HA-GRK6A was cotransfected with either empty vector or the indicated FLAG–NHERF1 construct into OKH cells. WT-NHERF1 coimmunoprecipitates with GRK6A, as does N1P2–GAGA– NHERF1 (PDZ1 intact). N1P1–GAGA (PDZ2 intact) and N1P1P2–GAGA/GAGA (both PDZ domains are modified) do not interact with NHERF1. B, NHERF1 coIP with GRK6A was quantified in transfected OKH cells and normalized to WT-NHERF1 (100%). N1P2 interacts with GRK6A to a similar extent to WT-NHERF1. Disruption of PDZ1 (N1P1–GAGA, N1P1P2–GAGA/GAGA) eliminated binding of GRK6A. Results report the mean ± SD (n = 3; ****p < 0.0001, ANOVA). coIP, coimmunoprecipitation; GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1.

Article Snippet: Chemically competent E. coli BL21 ΔSerB (AddGene #34929) cells were cotransformed with pCDF-1b Nherf1 PDZ2 (133–300) Ser162TAG 6xHis and pKW2 EF-Sep (25) and plated on LB agar plates containing 100 μg/ml spectinomycin and 25 μg/ml chloramphenicol.

Techniques: Western Blot, Plasmid Preparation, Construct, Transfection, Disruption, Binding Assay

Figure 7. General scheme represents the order of events along NHERF1-dependent PTH-sensitive phosphate uptake. NHERF1 PDZ1 binds NPT2A through the C-terminal (-TRL) motif and that keeps NPT2A at the apical membrane. PTH-induced phosphorylation promotes phosphorylation of Ser162 by PKCα. GRK6A binds pSer162−PDZ2 through the C-terminal (-TRL) motif and phosphorylates Ser290. NPT2A dissociates from NHERF1 and internalizes. GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1; PTH, parathyroid hormone.

Journal: Journal of Biological Chemistry

Article Title: Multisite NHERF1 phosphorylation controls GRK6A regulation of hormone-sensitive phosphate transport

doi: 10.1016/j.jbc.2021.100473

Figure Lengend Snippet: Figure 7. General scheme represents the order of events along NHERF1-dependent PTH-sensitive phosphate uptake. NHERF1 PDZ1 binds NPT2A through the C-terminal (-TRL) motif and that keeps NPT2A at the apical membrane. PTH-induced phosphorylation promotes phosphorylation of Ser162 by PKCα. GRK6A binds pSer162−PDZ2 through the C-terminal (-TRL) motif and phosphorylates Ser290. NPT2A dissociates from NHERF1 and internalizes. GRK6A, G protein–coupled receptor kinase 6A; NHERF1, Na+/H+ exchange regulatory factor-1; PTH, parathyroid hormone.

Article Snippet: Chemically competent E. coli BL21 ΔSerB (AddGene #34929) cells were cotransformed with pCDF-1b Nherf1 PDZ2 (133–300) Ser162TAG 6xHis and pKW2 EF-Sep (25) and plated on LB agar plates containing 100 μg/ml spectinomycin and 25 μg/ml chloramphenicol.

Techniques: Membrane, Phospho-proteomics